anti rabbit txred  (Vector Laboratories)

Journal: Cerebrospinal Fluid Research

Article Title: Hydrocephalus induces dynamic spatiotemporal regulation of aquaporin-4 expression in the rat brain

doi: 10.1186/1743-8454-7-20

Figure Lengend Snippet: Double immunofluorescence for aquaporin-4 and glial fibrillary acidic protein in control and two week hydrocephalic rat . Aquaporin-4 is visualized with TxRED (red) and glial fibrillary acidic protein with FITC (green). Nuclear counterstaining with DAPI (blue). Pictures represent glia limitans (A-F), periventricular region (including ependyma) (G-L) and vessels (M-R). Yellow colours in merged pictures confirm co-localization of aquaporins-4 and astrocytic GFAP in glia limitans and in perivascular endfeet. Both in control and in all hydrocephalic groups aquaporin-4 and glial fibrillary acidic protein showed co-localisation in the astroglial endfeet surrounding the vessels (M-R) and in glia limitans (A-F). Astrocytic cell soma were mainly without evidence of aquaporin-4 expression. * = lateral ventricle. Scale bar 100 μm.

Article Snippet: The primary anti-AQP4 antibodies were detected by using goat anti-rabbit IgG 1:50 linked with Texas red (TxRED) (Southern Biotech, Birmingham, USA, cat. no.: 4050-07), while the anti-GFAP antibodies were detected by using goat anti-rat IgG linked with fluorescein isothiocyanate (FITC) (Calbiochem, The Merck Group, Nottingham, United Kingdom, cat.no.

Techniques: Immunofluorescence, Control, Expressing